{"product_id":"abcam-ab246536","title":"Abcam, ab246536, Human 4-1BBL ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests \/ 10 x 96Tests\u003cbr\u003e\nHuman 4-1BBL ELISA Kit is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify Human 4-1BBL with a sensitivity of 2.2 pg\/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric assay - 450 nm readout - works on any plate reader - Different formats for different needs: 10x 96-well plates for bulk orders and 384-well for higher throughput - Need to design your own assay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Citrate plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 2.2 pg\/mL,\u003cbr\u003e\nRange:6.25 - 400 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman 4-1BBL ELISA Kit ab246536 is a rapid single-wash 90-min ELISA kit to measure Human 4-1 BBL in Citrate plasma, Cell culture supernatant, Serum. This SimpleStep ELISA® sensitivity is 5.64 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman 4-1 BBL SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit is designed for the quantification of Human 4-1BBL. The standard protein in this kit is the extracellular domain of Human 4-1BBL. Native signal was detected in cell culture supernatant. Spiked protein experiments were used to validate serum and plasma (citrate) sample types. Neat pooled serum and plasma (citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay. Saliva, urine, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit. This kit is incompatible with plasma (EDTA) and plasma (heparin) samples.\u003cbr\u003e\nHuman 4-1BBL ELISA Kit ab246536 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Human 4-1BBL ELISA Kit only requires a maxmum of 12.5µL\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation\u003cbr\u003e\n- Human 4-1BBL Antibody Pair - BSA and Azide free\u003cbr\u003e\nab256681\u003cbr\u003e\n- Anti-4-1BBL antibody [EPR21995-69] - BSA and Azide free (Capture)\u003cbr\u003e\nab259609\u003cbr\u003e\n- Anti-4-1BBL antibody [EPR21995-47] - BSA and Azide free (Detector)\u003cbr\u003e\nab259610\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman 4-1BBL ELISA Kit ab246536 is commonly used to study immune cell activation and regulation:\u003cbr\u003e\nOther kits for measuring immune response include:\u003cbr\u003e\n- Human CD40L ELISA kit\u003cbr\u003e\nab99991\u003cbr\u003e\n- Human TNFSF4 ELISA kit (OX40L)\u003cbr\u003e\nab213842\u003cbr\u003e\n- Human TNF alpha ELISA Kit\u003cbr\u003e\nab181421\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843576844457,"sku":"ab246536","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab246536","provider":"Iright","version":"1.0","type":"link"}