{"product_id":"abcam-ab247744","title":"Abcam, ab247744, Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal TrkA antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR1104(2),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab247744 is the carrier-free version of\u003cbr\u003e\nab109010\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTrkA also known as neurotrophic tyrosine receptor kinase 1 (NTRK1) and TrkB known as neurotrophic tyrosine receptor kinase 2 (NTRK2) are part of the tropomyosin receptor kinase family. TrkA has a mass of approximately 140 kDa while TrkB weighs around 145 kDa. These proteins express mainly in the nervous system including the brain and peripheral tissues. TrkA binds primarily to nerve growth factor (NGF) while TrkB interacts with brain-derived neurotrophic factor (BDNF) and neurotrophin-4\/5 (NT-4\/5). They are important for neuronal development and function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTrkA and TrkB proteins promote survival differentiation and maintenance of specific neuronal populations. TrkA is involved in neuronal survival by dimerizing upon ligand binding activating downstream signaling pathways. This activity ensures proper neuronal function and structure. TrkB in addition to supporting neuronal differentiation also influences synaptic plasticity. Both act as receptors in the neurotrophin signaling pathway forming complexes with other intracellular proteins. These actions make TrkA and TrkB fundamental for proper nervous system development and response to damage.\u003cbr\u003e\nPathways\u003cbr\u003e\nTrkA and TrkB are central to the MAPK\/ERK and PI3K\/Akt signaling pathways. The MAPK\/ERK pathway influences cell growth and differentiation where TrkA activates Ras proteins leading to phosphorylation cascades. Meanwhile in the PI3K\/Akt pathway activation of TrkB affects cell survival and apoptosis often involving interactions with proteins like Shc and PLCγ. These pathways are significant for neuronal cell fate decisions highlighting TrkA and TrkB’s interaction with other pathway components for maintaining cellular homeostasis.\u003cbr\u003e\nTrkA and TrkB are linked to neurodegenerative diseases and cancers. Altered TrkA function or expression is associated with Alzheimer’s disease where downstream effects involve dysregulation of p75NTR a related protein. TrkB variations have been connected to psychiatric disorders such as depression. In some cancers increased expression or mutations in the TrkA or TrkB genes lead to tumorigenesis highlighting their importance in disease pathology. Understanding these connections aids in developing targeted therapies for these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850940371113,"sku":"ab247744","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab247744","provider":"Iright","version":"1.0","type":"link"}