{"product_id":"abcam-ab248435","title":"Abcam, ab248435, Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Lamin-A\/C antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR4520-11,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab248435 is the carrier-free version of\u003cbr\u003e\nab133269\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCleaved Lamin A + C (Asp230) also known as Progerin refers to a post-translationally modified form of A-type lamins that results from caspase-mediated cleavage at Asp230. The mass of Lamin A is approximately 74 kDa while Lamin C is around 65 kDa. These proteins mainly localize to the nuclear lamina a structural layer beneath the inner nuclear membrane. They express in a variety of tissues but are particularly abundant in cells with a high demand for nuclear structural integrity such as skin heart and muscle tissues.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThese cleaved lamins play a pivotal role in maintaining nuclear shape and stability. They serve as integral components of the nuclear lamina which is part of a complex scaffold that provides mechanical support to the nucleus. Cleaved Lamin A + C also aids in chromatin organization influencing gene expression. Their function impacts key cellular processes like DNA replication and repair as well as regulation of the cell cycle.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of Cleaved Lamin A + C is important within apoptosis and cellular senescence pathways. They interact with proteins such as Caspase-6 and Caspase-7 in these processes. Lamin cleavage leads to nuclear disassembly during programmed cell death while in cellular senescence it signals alterations in nuclear integrity that influence aging-related pathways.\u003cbr\u003e\nCleaved Lamin A + C associates with accelerated aging disorders such as Hutchinson-Gilford Progeria Syndrome (HGPS). In this condition the accumulation of mutated lamin proteins results in defective nuclear architecture. Disorders like muscular dystrophy present a link as well where abnormal proteins like Emerin and Nesprin could interact with lamins affecting muscle cell viability and function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46852535058601,"sku":"ab248435","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab248435","provider":"Iright","version":"1.0","type":"link"}