{"product_id":"abcam-ab248586","title":"Abcam, ab248586, Anti-HACE1 antibody [EPR7962] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal HACE1 antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR7962,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab248586 is the carrier-free version of\u003cbr\u003e\nab133637\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHACE1 also known as HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 is a protein with a mass of approximately 111 kDa. This protein functions as an E3 ubiquitin ligase facilitating the attachment of ubiquitin molecules to specific protein substrates marking them for degradation via the proteasome pathway. It achieves this by recognizing and binding to substrates through its ankyrin repeat domains and catalyzing the conjugation process using its HECT domain. HACE1 is ubiquitously expressed in various tissues including the brain kidney and heart indicating its broad cellular importance.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHACE1 plays a role in regulating cellular processes such as apoptosis cell cycle and oxidative stress response contributing to normal cellular homeostasis. It impacts signaling pathways by tagging proteins involved in these processes for degradation ensuring proper cell function and survival. HACE1 functions as part of a ubiquitin-proteasome complex that finely tunes cell signaling cascades through selective protein turnover. Its activity helps maintain a balanced level of critical proteins within the cell influencing cellular proliferation and survival.\u003cbr\u003e\nPathways\u003cbr\u003e\nSeveral important cellular processes involve HACE1 including the oxidative stress response and cell cycle regulation pathways. Within these pathways HACE1 interacts with proteins like RAC1 a small GTPase associated with ROS production and cellular growth control. By modulating RAC1 and other related proteins HACE1 contributes to maintaining the redox balance and ensuring proper cell cycle progression. This interaction highlights HACE1's role in safeguarding cellular integrity under variable conditions.\u003cbr\u003e\nHACE1 has been linked to Wilms' tumor and neurodegenerative diseases. In cancer the loss of HACE1 function results in unchecked cellular proliferation and tumor development marking it as a potential tumor suppressor. The protein's downregulation or mutation is often observed in Wilms' tumor where it interacts with pathways involving TP53 another significant tumor suppressor. In the context of neurodegenerative diseases HACE1's role in managing oxidative stress suggests a connection with disorders such as Parkinson’s disease where oxidative damage is a critical pathogenic factor.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46852285169833,"sku":"ab248586","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab248586","provider":"Iright","version":"1.0","type":"link"}