{"product_id":"abcam-ab249003","title":"Abcam, ab249003, Anti-Isoleucyl tRNA synthetase antibody [EPR10266(B)] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Isoleucyl tRNA synthetase antibody. Carrier free. Suitable for ICC\/IF, WB and reacts with Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR10266(B),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab249003 is the carrier-free version of\u003cbr\u003e\nab151557\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nIsoleucyl tRNA synthetase also known as IARS or IleRS is an essential enzyme that charges tRNA molecules with isoleucine. This enzyme weighs approximately 126 kDa and is part of the aminoacyl-tRNA synthetase family. By catalyzing the attachment of isoleucine to its corresponding tRNA it ensures the correct translation of mRNA into proteins. IARS is ubiquitously expressed in the cytoplasm of various cell types and tissues playing a critical role in protein synthesis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nIARS plays an important role in maintaining cellular homeostasis by ensuring the fidelity of protein translation. It forms part of a larger complex of aminoacyl-tRNA synthetases known for its multi-enzyme complex formation which assists in proper protein synthesis. Through its function in aminoacylation IARS maintains the balance necessary for cellular activity and division reflecting its importance across multiple biological processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nIARS has important responsibilities in the translation process which is a fundamental pathway in gene expression. In the context of translation the enzyme contributes to the aminoacyl-tRNA biosynthesis pathway often interacting with other tRNA synthetases to stabilize protein synthesis. Additionally IARS can relate to the mTOR signaling pathway indicating its connection with nutrient-sensing mechanisms and its role in regulating cell growth and proliferation.\u003cbr\u003e\nAltered IARS function has connections to various neurological disorders such as CARS-related Perrault Syndrome where it may interact with cysteinyl-tRNA synthetase (CARS). Additionally mutations in IARS can relate to liver disorders like Vanishing White Matter (VWM) disease where it is associated with endoplasmic reticulum stress pathways and connected proteins such as eIF2B. Understanding these interactions helps in designing therapeutic strategies targeting the protein for treatment interventions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851851452585,"sku":"ab249003","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab249003","provider":"Iright","version":"1.0","type":"link"}