{"product_id":"abcam-ab250398","title":"Abcam, ab250398, Anti-ERP27 antibody [EPR12503] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal ERP27 antibody. Carrier free. Suitable for ICC\/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR12503,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WB, ICC\/IF, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:AB250398 specifically detects a band only in pancreas tissue and not in other tissues.  The larger band size observed in mouse and rat tissues compared to human tissue may be due to ERP having only one glycosylation site in humans versus two in mouse and rat.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250398 is the carrier-free version of\u003cbr\u003e\nab181172\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nERP27 also known as Endoplasmic Reticulum Protein 27 is a protein component with a molecular mass of approximately 27 kDa. It resides within the endoplasmic reticulum (ER) of the cell where it participates in protein folding and processing. Expressed in many tissues ERP27 is part of the protein disulfide isomerase (PDI) family. Although its structural features do not include the typical thioredoxin-like active sites ERP27 influences protein maturation by assisting in the correct assembly and folding of nascent proteins.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe protein exhibits important roles in the cellular stress response and quality control mechanisms within the endoplasmic reticulum. It does not form a stable complex with other proteins but interacts transiently to exert its function. ERP27 collaborates closely with other ER-resident proteins like ERP44 and the PDI family to enhance the checking and refolding of misfolded proteins maintaining cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nERP27 participates directly in the unfolded protein response (UPR) pathway a critical mechanism activated during ER stress. This pathway involves ERP27's transient interactions within a network of proteins including ER chaperones and other PDIs to alleviate stress and restore normal function. Furthermore ERP27 plays a role in calcium homeostasis through these pathways interacting with proteins such as calnexin and calreticulin.\u003cbr\u003e\nERP27's impairment or dysregulation associates with neurodegenerative diseases like Alzheimer's and Parkinson’s. The protein's involvement in the UPR links it to cellular processes that are disrupted in these conditions. Moreover it connects to proteins such as amyloid precursor protein (APP) in Alzheimer's influencing amyloid-beta peptide production. In Parkinson’s interactions with parkin and related proteins highlight ERP27's potential implications in disease pathogenesis and progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46856179810473,"sku":"ab250398","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab250398","provider":"Iright","version":"1.0","type":"link"}