{"product_id":"abcam-ab250646","title":"Abcam, ab250646, Anti-Scc4 antibody [EPR14390] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal SCC4 antibody. Carrier free. Suitable for ICC\/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR14390,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WB, IP, ICC\/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250646 is the carrier-free version of\u003cbr\u003e\nab183033\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nScc4 also known as STAG2 or SCC4 is a subunit of the cohesion complex loading onto chromosomes during the cell division. It has a molecular weight of around 92 kDa. Scc4 is mainly expressed in the nucleoplasm and interacts with other cohesion subunits to help establish sister chromatid cohesion. It forms a complex with other proteins to ensure correct chromosome segregation which is an important process for maintaining genome stability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nScc4 plays a role in chromosomal dynamics and is part of the cohesion loader complex which includes Scc2. Scc4's main function involves facilitating the loading and stabilization of the cohesin complex on chromosomes during the S-phase of the cell cycle. Successfully forming the cohesion structure enables physical connection of sister chromatids ensuring they divide properly. This is important for genetic information to pass accurately from cell generation to generation.\u003cbr\u003e\nPathways\u003cbr\u003e\nScc4 is mostly involved in cell cycle regulation engaging especially in the pathways tied to chromosome segregation and DNA repair. The complex it forms with Scc2 is critical to load cohesins onto chromatin during DNA replication influencing other proteins like Scc1 and Rad21. These proteins coordinate cohesin activity and interaction in the DNA damage repair mechanisms alongside chromosome segregation processes.\u003cbr\u003e\nMutations in Scc4's gene have been linked to Cornelia de Lange Syndrome and certain forms of cancer especially acute myeloid leukemia. Its dysfunction can lead to improper chromosomal segregation and mutagenesis contributing to cancer development. The relationship between Scc4 and SMC3 or NIPBL other components of the cohesion complex highlights the interconnectedness of these proteins in both genetic stability and disease pathogenesis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46856159461545,"sku":"ab250646","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab250646","provider":"Iright","version":"1.0","type":"link"}