{"product_id":"abcam-ab250887","title":"Abcam, ab250887, Anti-EAAT4 antibody [EPR7742] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal EAAT4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR7742,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250887 is the carrier-free version of\u003cbr\u003e\nab186435\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEAAT4 also known as Excitatory Amino Acid Transporter 4 or SLC1A6 functions as a sodium-dependent glutamate transporter primarily facilitating the reuptake of glutamate into presynaptic neurons and glial cells. EAAT4 has an approximate molecular mass of 65 kDa. Expression occurs mainly in Purkinje cells within the cerebellum but it can also be found in other parts of the central nervous system albeit at lower levels.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEAAT4 controls extracellular glutamate concentration preventing excitotoxicity in neural circuits. EAAT4 forms part of the larger family of high-affinity glutamate transporters which includes EAAT1 to EAAT5. This transporter operates by coupling glutamate transport with the influx of sodium and the efflux of potassium and protons thereby maintaining ion balance. Although single in its action EAAT4 works alongside related transporters for comprehensive glutamate regulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nEAAT4 plays a significant role in glutamatergic signaling and cerebellar function. The transporter deeply integrates into the synaptic transmission pathway ensuring glutamate homeostasis critical for synaptic plasticity. EAAT4 works in functional tandem with proteins like EAAT3 facilitating efficient glutamate clearance and modulating excitatory signaling within neuronal pathways.\u003cbr\u003e\nEAAT4 relates to neurodegenerative conditions where disrupted glutamate homeostasis plays a part such as Amyotrophic Lateral Sclerosis (ALS) and Spinocerebellar Ataxia. Excessive extracellular glutamate can lead to neuronal damage linking EAAT4 function with these pathologies. Moreover EAAT4 interacts with proteins like GLT-1 in these disease contexts to attempt to mitigate glutamatergic dysfunction.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46852379902121,"sku":"ab250887","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab250887","provider":"Iright","version":"1.0","type":"link"}