{"product_id":"abcam-ab250931","title":"Abcam, ab250931, Anti-MCAK antibody [EPR14838] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal MCAK antibody. Carrier free. Suitable for ICC\/IF, WB, Flow Cyt (Intra) and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR14838,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, Flow Cyt (Intra), ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250931 is the carrier-free version of\u003cbr\u003e\nab187652\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe MCAK protein also known as KIF2C (kinesin family member 2C) is a motor protein that makes depolymerization of microtubules. It serves an important role in the regulation of microtubule dynamics during mitosis. MCAK has a molecular mass of approximately 81 kDa. Expression of MCAK is mostly found in actively dividing cells such as those in tissues with high turnover rates including the skin intestinal lining and specific tumors. This protein is critical for the proper formation and functioning of the mitotic spindle during cell division.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe MCAK protein contributes to accurate chromosome segregation. MCAK performs its functions by binding to microtubule ends and removing tubulin subunits thereby aiding in microtubule disassembly. It functions within the mitotic spindle apparatus and ensures that chromosomes align properly and evenly separate to opposite poles of the dividing cell. This activity is not part of a complex but acts independently to correct improper microtubule attachments.\u003cbr\u003e\nPathways\u003cbr\u003e\nMCAK plays a significant role in mitotic and cell cycle pathways. It coordinates with other proteins like Aurora B kinase which is involved in correcting improper kinetochore-microtubule attachments to maintain chromosome stability during mitosis. MCAK interacts with proteins controlling cell cycle checkpoints overlapping with pathways ensuring cells do not progress with errors in chromosome segregation.\u003cbr\u003e\nAberrant regulation of the MCAK protein can lead to chromosomal instability often observed in cancers such as colorectal and breast cancer. This instability can drive tumorigenesis where loss of proper chromosome segregation leads to aneuploidy and cancer progression. In these cancers MCAK may interact with other proteins such as Aurora kinases revealing potential therapeutic targets for intervention in malignancies with defective mitotic processes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46856019116201,"sku":"ab250931","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab250931","provider":"Iright","version":"1.0","type":"link"}