{"product_id":"abcam-ab251153","title":"Abcam, ab251153, Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal MAT2A antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR19048,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab251153 is the carrier-free version of\u003cbr\u003e\nab192846\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMAT1A and MAT2A are enzymes also known as methionine adenosyltransferase 1A and 2A which play a critical role in the synthesis of S-adenosylmethionine (SAMe). MAT1A has a molecular mass of about 42 kDa while MAT2A varies slightly due to post-translational modifications. These enzymes are mainly expressed in the liver and extrahepatic tissues respectively. MAT1A predominantly functions in the liver where it serves an essential part in maintaining normal liver metabolism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMethionine adenosyltransferase 1A and 2A function to catalyze the transfer of an adenosyl group from ATP to methionine forming SAMe. This reaction is part of a critical metabolic process acting as a source of methyl groups for methylation reactions. MAT1A commonly works as part of a homotetrameric complex while MAT2A can form both dimers and tetramers. This flexibility in complex formation helps regulate the production of SAMe based on cellular demands linking it to vital biomolecules synthesis.\u003cbr\u003e\nPathways\u003cbr\u003e\nMethionine salvage pathways and polyamine biosynthesis include the significant involvement of MAT1A and MAT2A. These enzymes provide the necessary SAMe for the transmethylation and transsulfuration pathways connecting to other proteins such as GNMT and SAH hydrolase. The enzymes' activity influences the levels of important intermediates impacting these pathways and resulting metabolic outputs.\u003cbr\u003e\nAbnormal expression or dysfunction of methionine adenosyltransferase 1A and 2A associates with liver cirrhosis and hepatocellular carcinoma. Alterations in the enzymes’ activity can disrupt normal SAMe levels affecting methylation reactions and subsequent gene expression patterns. Key proteins like cyclin-dependent kinase (CDK) also interact with these enzymes further linking them to cancer progression and outcomes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46856370389161,"sku":"ab251153","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab251153","provider":"Iright","version":"1.0","type":"link"}