{"product_id":"abcam-ab252895","title":"Abcam, ab252895, Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nIn Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric) (ab252895), FAAH hydrolyzes a non-fluorescent substrate releasing 7-amino-4-methylcoumarin (AMC), a fluorophore, which can be easily measured at Ex\/Em= 360\/465 nm.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Microsomes, Tissue Homogenate, Adherent cells,\u003cbr\u003e\nSensitivity:= 0.1 µU,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nIn Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric) (ab252895), FAAH hydrolyzes a non-fluorescent substrate releasing 7-amino-4-methylcoumarin (AMC), a fluorophore, which can be easily measured at Ex\/Em= 360\/465 nm. The kit provides a specific inhibitor that can be used to compensate for potential non-specfic background in unknown samples. The stable fluorescence signal is positively correlated to FAAH enzymatic activity in samples. The kit offers a rapid, simple, sensitive, reproducible assay and is suitable for detecting FAAH activity as low as 0.1 μU.\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K434 Fatty Acid Amide Hydrolase (FAAH) Activity Assay Kit (Fluorometric). K434-100 is the same size as the 100 test size of ab252895.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nFatty acid amide hydrolase 1 (FAAH1) often referred to simply as FAAH is an enzyme that plays a critical role in the degradation of fatty acid amides such as anandamide in the body. This enzyme weighing approximately 63 kDa is a part of the amidase signature family and is characterized by its amidase hydrolase activity. FAAH1 is predominantly expressed in the nervous system liver and gastrointestinal tract where it hydrolyses amide bonds facilitating the termination of signaling pathways initiated by neurotransmitter-like fatty acid amides.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nFAAH1 is essential for maintaining homeostasis by modulating levels of bioactive lipids. It exerts control over endocannabinoid signaling by breaking down anandamide a lipid derivative functioning as a neurotransmitter. FAAH1 does not act as part of a larger protein complex. Instead it operates alone to regulate the duration and intensity of signaling rather than relying on direct interactions within a complex. The enzyme's function impacts the neurochemical milieu in tissues where it is actively expressed thereby affecting mood pain sensation and more.\u003cbr\u003e\nPathways\u003cbr\u003e\nFAAH1 has a significant role in the endocannabinoid signaling pathway and fatty acid metabolism. Within these pathways it modulates signaling by hydrolysing bioactive amides like anandamide influencing overall lipid signaling. FAAH1 activities intersect with several other proteins such as cannabinoid receptors which bind the substrates that FAAH1 eventually degrades. These interactions are vital in controlling signal transduction processes that regulate physiological responses including stress and analgesia.\u003cbr\u003e\nFAAH1 has been implicated in conditions like chronic pain and anxiety disorders. Altered FAAH1 function or expression can lead to dysregulation of endocannabinoid signaling contributing to the pathophysiology of these conditions. The enzyme's modulation of anandamide levels connects it to proteins such as CB1 and CB2 cannabinoid receptors which mediate the effects of endocannabinoids in neurological and psychiatric disorders. Research into FAAH1 inhibitors as therapeutic agents highlights its importance in potential treatments for managing pain and anxiety.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843577827497,"sku":"ab252895","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab252895","provider":"Iright","version":"1.0","type":"link"}