{"product_id":"abcam-ab252899","title":"Abcam, ab252899, Tyrosinase Activity Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nTyrosinase Activity Assay Kit (Colorimetric) (ab252899) is a mix-and-read, one-step, plate-based assay for the measurement of tyrosinase activity in biological samples. Readout on any (510 nm) colorimetric plate reader.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Purified protein, Tissue Lysate, Tissue Homogenate, Cell Lysate,\u003cbr\u003e\nAssay type:Enzyme activity (quantitative),\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nTyrosinase Activity Assay Kit (Colorimetric) (ab252899) is a simple one-step, plate-based assay for the measurement of tyrosinase activity in biological samples. In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone intermediate, which reacts with the tyrosine enhancer forming a highly stable chromophore with absorbance at 510 nm. The assay can detect as low as 30 μU Tyrosinase in biological samples.\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K742 Tyrosinase Activity Assay Kit (Colorimetric). K742-100 is the same size as the 100 test size of ab252899.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTyrosinase also known as monophenol monooxygenase is a copper-containing enzyme with a mass of approximately 60-70 kDa. It catalyzes the oxidation of phenols such as tyrosine to quinones which are important precursors for melanin synthesis. Tyrosinase is predominantly expressed in melanocytes the cells responsible for pigment production in skin hair and eyes. Its expression also occurs in other tissues like the brain where it might play different roles.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nWithin melanin production tyrosinase functions as the rate-limiting enzyme initiating the synthesis path by converting tyrosine into dopaquinone. This enzyme works as part of the melanogenic complex interacting with other enzymes such as tyrosinase-related proteins 1 and 2 to regulate melanin synthesis. The presence and activity of tyrosinase significantly impacts pigmentation processes and skin coloration.\u003cbr\u003e\nPathways\u003cbr\u003e\nRelating tyrosinase to melanin biosynthetic and regulatory pathways reveal its central role. The enzyme is vital in the Raper-Mason pathway for melanin production where it interacts with proteins such as dopachrome tautomerase. It bridges the gap between external stimuli like UV radiation and cellular responses transforming them into pigmentation changes. The L-tyrosine and catecholamine biosynthesis pathways also influence tyrosinase activity linking it to broader metabolic processes.\u003cbr\u003e\nIssues with tyrosinase can lead to conditions like albinism and melanoma. In oculocutaneous albinism type 1 (OCA1) a genetic mutation in the tyrosinase gene results in reduced or absent enzymatic activity causing hypopigmentation. In melanoma abnormal regulation of tyrosinase and its pathway associates with tumor progression and metastasis often in connection with factors such as microphthalmia-associated transcription factor (MITF) that modulate its expression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843629207721,"sku":"ab252899","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab252899","provider":"Iright","version":"1.0","type":"link"}