{"product_id":"abcam-ab253508","title":"Abcam, ab253508, Human Insulin Antibody Pair - BSA and Azide free","description":"\u003cp\u003eSize: 10 x 96Tests\u003cbr\u003e\nHuman Insulin Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Insulin.\u003cbr\u003e\nKey facts\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:ELISA set,\u003cbr\u003e\nRange:25.56 - 425 pg\/mL,\u003cbr\u003e\nAssay Platform:Reagents\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Insulin Antibody Pair is a matched pair of unconjugated\u003cbr\u003e\nrecombinant rabbit monoclonal\u003cbr\u003e\ncapture and detection antibodies used to quantify Human Insulin in sandwich ELISAs and many other pair-based applications.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nUse our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with \u0026lt;1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nThe pair can be used in variety of assays and platforms including but not limited to:\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nFRET\/TR-FRET\/HTRF\u003cbr\u003e\nMeso Scale Discovery\u003cbr\u003e\nBead-based assays\u003cbr\u003e\nAlphaLISA\u003cbr\u003e\n\/AlphaScreen\u003cbr\u003e\nDELFIA\u003cbr\u003e\nimmunoassays\u003cbr\u003e\nand Single Molecule Counting (SMC™) immunoassays\u003cbr\u003e\nMultiplex\u003cbr\u003e\nOur antibody pairs are supplied in a carrier-free format that is conjugation-ready:\u003cbr\u003e\nBuffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.\u003cbr\u003e\nConcentration of ~1 mg\/ml as measured by the protein A280 method.\u003cbr\u003e\nWe can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.\u003cbr\u003e\nPairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.\u003cbr\u003e\nPlease note:\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.\u003cbr\u003e\nThe range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.\u003cbr\u003e\nWe guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.\u003cbr\u003e\nAntibody properties:\u003cbr\u003e\nCapture antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) – 100 µg\u003cbr\u003e\nDetector antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) - 100 µg\u003cbr\u003e\nConcentration\u003cbr\u003e\n: ~1 mg\/ml\u003cbr\u003e\nStorage buffer:\u003cbr\u003e\n100% PBS\u003cbr\u003e\nLiquid\u003cbr\u003e\nIsotype:\u003cbr\u003e\nRecombinant monoclonal antibodies offer several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free production\u003cbr\u003e\nMeso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.\u003cbr\u003e\nAlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.\u003cbr\u003e\nSimoa is a registered trademark of Quanterix, Inc.\u003cbr\u003e\nSMC is a registered trademark of Merck KGaA, Darmstadt, Germany.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nInsulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nInsulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.\u003cbr\u003e\nPathways\u003cbr\u003e\nInsulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.\u003cbr\u003e\nInsulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843601453225,"sku":"ab253508","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab253508","provider":"Iright","version":"1.0","type":"link"}