{"product_id":"abcam-ab253569","title":"Abcam, ab253569, Human SGK1 Antibody Pair - BSA and Azide free","description":"\u003cp\u003eSize: 10 x 96Tests\u003cbr\u003e\nHuman SGK1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human SGK1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:ELISA set,\u003cbr\u003e\nRange:0.31 - 40 ng\/mL,\u003cbr\u003e\nAssay Platform:Reagents\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman SGK1 Antibody Pair is a matched pair of unconjugated\u003cbr\u003e\nrecombinant rabbit monoclonal\u003cbr\u003e\ncapture and detection antibodies used to quantify Human SGK1 in sandwich ELISAs and many other pair-based applications.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nUse our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with \u0026lt;1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nThe pair can be used in variety of assays and platforms including but not limited to:\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nFRET\/TR-FRET\/HTRF\u003cbr\u003e\nMeso Scale Discovery\u003cbr\u003e\nBead-based assays\u003cbr\u003e\nAlphaLISA\u003cbr\u003e\n\/AlphaScreen\u003cbr\u003e\nDELFIA\u003cbr\u003e\nimmunoassays\u003cbr\u003e\nand Single Molecule Counting (SMC™) immunoassays\u003cbr\u003e\nMultiplex\u003cbr\u003e\nOur antibody pairs are supplied in a carrier-free format that is conjugation-ready:\u003cbr\u003e\nBuffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.\u003cbr\u003e\nConcentration of ~1 mg\/ml as measured by the protein A280 method.\u003cbr\u003e\nWe can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.\u003cbr\u003e\nPairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.\u003cbr\u003e\nPlease note:\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.\u003cbr\u003e\nThe range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.\u003cbr\u003e\nWe guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.\u003cbr\u003e\nAntibody properties:\u003cbr\u003e\nCapture antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) – 100 µg\u003cbr\u003e\nDetector antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) - 100 µg\u003cbr\u003e\nConcentration\u003cbr\u003e\n: ~1 mg\/ml\u003cbr\u003e\nStorage buffer:\u003cbr\u003e\n100% PBS\u003cbr\u003e\nLiquid\u003cbr\u003e\nIsotype:\u003cbr\u003e\nRecombinant monoclonal antibodies offer several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free production\u003cbr\u003e\nMeso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.\u003cbr\u003e\nAlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.\u003cbr\u003e\nSimoa is a registered trademark of Quanterix, Inc.\u003cbr\u003e\nSMC is a registered trademark of Merck KGaA, Darmstadt, Germany.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Serum and Glucocorticoid-Regulated Kinase 1 also known as SGK1 is a protein kinase involved in cellular stress response. It consists of approximately 49 kDa and is encoded by the SGK1 gene. SGK1 is expressed in several tissues including the kidney pancreas and liver. It transfers phosphate groups from ATP to serine or threonine residues on target proteins functioning as an important regulator of ion channels enzymes and other cellular proteins.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSGK1 regulates ion transport cell proliferation and survival pathways. SGK1 is not usually part of large protein complexes but interacts dynamically with other proteins to exert its effects. It plays a major role in regulating sodium channels in the kidney therefore modulating sodium balance and blood pressure. It also affects various cell survival mechanisms influencing cell growth and apoptosis in response to physiological cues.\u003cbr\u003e\nPathways\u003cbr\u003e\nSGK1 is involved in the PI3K\/AKT\/mTOR signaling pathway and the WNK\/SPAK pathway. These pathways influence cellular growth metabolism and ion transport. SGK1 shares pathway relevance with proteins like AKT1 and mTOR in the PI3K\/AKT\/mTOR axis impacting various metabolic and growth-related cellular processes. In the WNK\/SPAK pathway SGK1 regulates ion channels in collaboration with WNK kinases affecting electrolyte balance.\u003cbr\u003e\nSGK1 has implications in hypertension and cancer. Overactivity of SGK1 can lead to increased sodium retention resulting in hypertension. This connects SGK1 to diseases involving blood pressure regulation where it often interacts with the epithelial sodium channel (ENaC). In cancer SGK1 contributes to tumor progression by supporting cell survival with interactions alongside AKT1 frequently observed in cancerous pathways. Alterations in SGK1 levels or activity may therefore serve as a therapeutic target for managing these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843606630569,"sku":"ab253569","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab253569","provider":"Iright","version":"1.0","type":"link"}