{"product_id":"abcam-ab254154","title":"Abcam, ab254154, Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nAnti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154) is a mouse monoclonal antibody detecting Detyrosinated alpha Tubulin in  Western Blot, IHC-P, IHC-Fr, ICC\/IF . Suitable for  Human, Mouse, Rat .\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:AA12,\u003cbr\u003e\nIsotype:IgG2a,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WB, IHC-Fr, IHC-P, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of Detyrosinated alpha Tubulin?\u003cbr\u003e\nAnti-Detyrosinated alpha Tubulin [AA12] - Microtubule Marker (ab254154) specifically detects a band for Detyrosinated alpha Tubulin (UniProt: Q71U36) at a molecular weight of 50kDa.\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDetyrosinated alpha Tubulin also known as detyrosinated tubulin or alpha tubulin is a modified form of the alpha subunit of tubulin. This protein undergoes detyrosination which entails removing the terminal tyrosine residue. Detyrosinated alpha Tubulin has an approximate molecular mass of 50 kDa. It localizes mainly in the cytoplasm and plays an important role in the stability and function of microtubules which are key components of the cytoskeleton. These modifications regulate microtubules' dynamic properties impacting cellular processes such as intracellular transport and cell division.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nDetyrosinated alpha Tubulin impacts important cellular functions. It is an integral part of the microtubule network. Microtubules provide structural support to the cell aiding in intracellular transport and cell shape maintenance. The detyrosination of alpha tubulin contributes to the regulation of microtubule stability which is essential for accurate chromosome segregation during mitosis. Furthermore the detyrosination influences interactions with microtubule-associated proteins and motor proteins affecting their binding affinity and activity.\u003cbr\u003e\nPathways\u003cbr\u003e\nDetyrosinated alpha Tubulin is significant in cellular signaling and transport. It is an important part of the microtubule-dependent transport pathways that facilitate the movement of cellular components including vesicles and organelles. The detyrosination of alpha tubulin influences its interaction with motor proteins such as kinesins and dyneins which are responsible for directional transport along microtubules. Additionally alterations in tubulin detyrosination affect the Wnt signaling pathway impacting cellular processes such as proliferation and differentiation.\u003cbr\u003e\nAlterations in the detyrosination of alpha Tubulin have links to neurodegenerative diseases and cancer. In neurodegenerative disorders abnormal detyrosination can disrupt axonal transport contributing to diseases like Alzheimer's. In cancer increased levels of detyrosinated alpha Tubulin can enhance cell invasiveness and metastasis. The dysregulation of tubulin detyrosination affects cellular interactions with proteins such as tau in neurodegenerative disorders and beta-catenin in cancer implicating these proteins in disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848442695849,"sku":"ab254154","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab254154","provider":"Iright","version":"1.0","type":"link"}