{"product_id":"abcam-ab254503","title":"Abcam, ab254503, Human Alkaline Phosphatase ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Alkaline Phosphatase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Alkaline Phosphatase with a sensitivity of 73 ng\/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 73 ng\/mL,\u003cbr\u003e\nRange:1.09 - 70 ng\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman ALPL ELISA Kit (ab254503) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALPL protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ALPL with 73 ng\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n- Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n- High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n- Fully validated in biological samples\u003cbr\u003e\n- 96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nALPL, also known as tissue-nonspecific alkaline phosphatase, catalyzes phosphate ester hydrolysis when in a basic environment. Deficiency in this protein leads to loss in mineralization of bone and teeth which can be fatal. ALPL is currently being used a prognostic marker for the treatment of metastatic castration-resistant prostate cancer.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlkaline Phosphatase Tissue Non-Specific also referred to as ALPL or alkaline phosphatase protein functions mechanically as a hydrolase enzyme. It catalyzes the dephosphorylation of molecules contributing to phosphate and mineral metabolism. This enzyme with a molecular mass of approximately 57 kDa exhibits expression in various tissues including liver bone and kidneys. Researchers can use techniques like alkaline phosphatase ELISA and immunohistochemistry to study its expression and localization.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAlkaline phosphatase in tissues plays a pivotal role in bone mineralization and development. It functions to hydrolyze phosphate groups releasing inorganic phosphate necessary for bone and teeth formation. It forms part of a larger enzyme complex that interacts with extracellular substrates ensuring the continuous supply of phosphate ions. This enzyme in particular impacts cellular processes involving calcification and cellular differentiation.\u003cbr\u003e\nPathways\u003cbr\u003e\nAlkaline phosphatase operates within phosphate metabolism and signaling pathways. Within the skeletal system it forms part of the regulatory mechanism for osteoblast activity and bone formation. It relates to proteins like osteopontin and bone sialoprotein which regulate the mineralization process. These pathways ensure the balance between phosphate ions and mineral deposition in bone tissue.\u003cbr\u003e\nAlkaline phosphatase abnormalities correlate with hypophosphatasia and rickets. Hypophosphatasia results from mutations in the ALPL gene causing defective bone mineralization. In cases of rickets improper phosphate metabolism can occur due to dysfunctional enzyme activity. The imbalance in alkaline phosphatase activity also connects to disorders like hyperparathyroidism where calcium and phosphate homeostasis is disrupted involving proteins such as parathyroid hormone.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843575435433,"sku":"ab254503","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab254503","provider":"Iright","version":"1.0","type":"link"}