{"product_id":"abcam-ab255329","title":"Abcam, ab255329, Anti-Amylin\/DAP antibody [EPR22556-138] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nAnti-Amylin\/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation. Suitable for  Flow Cytometry, IP, IHC-P, IHC-Fr, ICC\/IF  in  Human, Mouse, Rat . - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR22556-138,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), IHC-Fr, ICC\/IF, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Amylin\/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Mouse, Rat samples.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR22556-138] also available for your convenience:\u003cbr\u003e\nab254259\u003cbr\u003e\n, Carrier free - ab255329, PE -\u003cbr\u003e\nab306057\u003cbr\u003e\n, APC -\u003cbr\u003e\nab306058\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab306059\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321500\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAmylin also known as Islet Amyloid Polypeptide (IAPP) is a peptide hormone mainly produced and co-secreted with insulin by pancreatic beta cells. This peptide has a molecular mass of approximately 3.9 kDa. Amylin is highly expressed in the pancreas with some expression in the gastrointestinal tract and the central nervous system. In images DAP typically refers to 4'6-diamidino-2-phenylindole which isn't directly related to amylin but this clarification can avoid confusion in laboratory discussions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAmylin peptide regulates blood glucose levels by slowing gastric emptying and promoting satiety. It acts as part of a complex with calcitonin receptor and a receptor activity-modifying protein to mediate its effects efficiently. Amylin influences the central nervous system by affecting appetite and has roles in bone metabolism through interactions with receptors related to alkaline phosphatase 138.\u003cbr\u003e\nPathways\u003cbr\u003e\nAmylin functions in the glucose homeostasis and energy regulation pathways. It closely works with insulin impacting how the body manages postprandial glucose spikes. The peptide also interacts with cAMP signaling which is part of the same network that involves proteins like glucagon-like peptide-1 (GLP-1) which harmonizes action between different metabolic regulatory components.\u003cbr\u003e\nAmylin accumulates in the form of amyloid deposits in type 2 diabetes contributing to the degeneration of beta cells. The presence of amylin aggregates connects with insulin in this pathology aggravating disease progression. Moreover the peptide plays a part in Alzheimer's disease where its amyloidogenic properties may have overlapping mechanisms with amyloid beta deposits impacting neurodegeneration through similar receptor pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848517275817,"sku":"ab255329","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab255329","provider":"Iright","version":"1.0","type":"link"}