{"product_id":"abcam-ab255379","title":"Abcam, ab255379, Human COL4A1 (Collagen IV alpha 1) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nCOL4A1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 25. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 25,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-COL4A1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCollagen IV alpha 1 also known as COL4A1 forms an essential component of type IV collagen a major structural protein in basement membranes. With a molecular mass of approximately 185 kDa it contributes significantly to the formation of the structural matrix that supports epithelial and endothelial cells. Expression of COL4A1 is widespread notably in tissues with high involvement of basement membranes such as kidneys the eye and the cerebral vasculature. It takes part in the assembly of the collagen IV network essential for a stable extracellular environment.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCollagen type IV alpha 1 contributes to the structural integrity and mechanical stability of the basement membrane. It operates as part of a heterotrimeric complex alongside COL4A2 integrating into the basement membrane's unique mesh-like structure. This arrangement is important for the maintenance of cell attachment movement and the filtration barrier in tissues like the glomeruli. This protein also facilitates separations between tissue compartments helping organize cell layers into functional units.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe collagen type IV alpha 1 protein participates in the integrin signaling pathway and the matrix metalloproteinase (MMP) pathway. It interacts with other collagen forms and proteins such as integrins involved in signaling events that regulate cell survival proliferation and migration. Its involvement in the MMP pathway highlights its role in tissue remodeling and repair as MMPs modulate the extracellular environment including basement membrane degradation and reconstruction.\u003cbr\u003e\nMutations or abnormalities in collagen type IV alpha 1 relate to conditions like cerebral small vessel disease (SVD) and Alport syndrome. SVD is associated with compromised vascular integrity where COL4A1 mutations may disrupt normal blood vessel function. In Alport syndrome disruptions in type IV collagen may lead to progressive kidney disease due to the protein's vital role in glomerular basement membrane structure. Furthermore COL4A1's interaction with proteins like COL4A5 highlights its broader impact in these disorders as COL4A5 mutations are a known cause of Alport syndrome.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845081714857,"sku":"ab255379","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab255379","provider":"Iright","version":"1.0","type":"link"}