{"product_id":"abcam-ab256478","title":"Abcam, ab256478, Anti-eRF3\/GSPT1 antibody [EPR22908-103] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal eRF3\/GSPT1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Recombinant fragment - Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR22908-103,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab256478 is the carrier-free version of\u003cbr\u003e\nab234433\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe eRF3\/GSPT1 protein also known as GSPT1 plays an important role in translation termination. It works with eRF1 to release the newly synthesized polypeptide chain from the ribosome. GSPT1 is a GTPase of approximately 69 kDa commonly expressed in human tissues with higher concentrations found in proliferating cells such as those in the bone marrow and lymphoid tissues. It implements essential tasks in protein synthesis regulation by facilitating proper translation termination.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGSPT1 is involved in processes beyond translation termination. It associates with complexes that regulate mRNA translation and stability. As part of the eRF3 pathway GSPT1 interacts with various translation and post-translation machinery. Its molecular function extends to include potential interactions with lncRNAs such as lnc-SNHG16 indicating a role in cellular processes like cell growth and division. GSPT1's interaction network suggests that it performs multifunction in cell regulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nGSPT1 integrates into the translation termination pathway tightly linking with the mRNA surveillance pathway. This protein is essential for efficient recognition and release of stop codons. In these pathways it collaborates with proteins such as eRF1 to regulate mRNA stability and translation accuracy. These interactions ensure the fidelity of protein biosynthesis and proper cellular function.\u003cbr\u003e\nThe role of GSPT1 extends to cancer and neurodegenerative diseases. Overexpression or mutations of GSPT1 links to various cancers including gastric and colorectal cancers where it affects cell proliferation pathways. The GSPT1-Myc axis appears to contribute significantly to oncogenesis acting with proteins like c-Myc to promote tumorigenesis. Additionally disruptions in GSPT1 function could associate with neurological disorders where translation termination processes malfunction although these aspects require further study.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46852513431721,"sku":"ab256478","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab256478","provider":"Iright","version":"1.0","type":"link"}