{"product_id":"abcam-ab257688","title":"Abcam, ab257688, Human SMARCB1 (SNF5) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSMARCB1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon2 and 1 bp insertion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon2 and 1 bp insertion in exon2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SMARCB1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSNF5\/SMARCB1 also known as INI1 or BAF47 is a member of the SWI\/SNF chromatin remodeling complex. It acts in modifying chromatin structure to regulate gene expression. SNF5\/SMARCB1 possesses a mass of approximately 47 kDa. The protein expresses ubiquitously in various tissues with notable presence in the nucleus. Through its mechanical activity in chromatin remodeling SNF5\/SMARCB1 facilitates transcriptional regulation and chromosomal stability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSNF5\/SMARCB1 acts as an integral component of the SWI\/SNF complex. This complex functions in altering nucleosome positioning on DNA impacting gene expression linked to cell cycle regulation differentiation and DNA repair. By working within this complex SNF5\/SMARCB1 helps in the activation or repression of specific gene sets based on cellular requirements displaying an essential role in maintaining normal cellular function. The interplay of SNF5\/SMARCB1 with other chromatin-modifying enzymes extends its influence across various genetic pathways.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of SNF5\/SMARCB1 in the cell cycle and transcription regulation pathways is acknowledged. It participates intimately in the p53 signaling pathway which is critical for cell cycle checkpoints and apoptotic responses. SNF5\/SMARCB1 engages with other proteins such as p53 and RB1 coordinating to maintain genomic integrity and proper transcriptional programming. These pathways spotlight the protein's influence in important cell fate decisions.\u003cbr\u003e\nSNF5\/SMARCB1 mutations or deletions associate closely with malignant rhabdoid tumors an aggressive pediatric cancer and schwannomatosis a disorder involving benign nerve sheath tumors. The loss of SNF5\/SMARCB1 disrupts the SWI\/SNF complex's function triggering unregulated cell proliferation and cancer progression. Within these disorders the SWI\/SNF complex itself which contains other subunits like BRG1 and BRM is also impacted highlighting SMARCB1's critical role in tumor suppression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845073096873,"sku":"ab257688","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab257688","provider":"Iright","version":"1.0","type":"link"}