{"product_id":"abcam-ab257723","title":"Abcam, ab257723, Human TACC3 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTACC3 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 4.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 4.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TACC3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTACC3 also known as Transforming Acidic Coiled-Coil Containing Protein 3 is a microtubule-associated protein with a molecular weight of approximately 90 kDa. It plays an important role in stabilizing microtubules and is involved in mitotic spindle assembly. TACC3 is expressed in various tissues with high levels seen in proliferating cells such as embryonic cells and those in the testis. This expression pattern suggests it has important functions during cell division and development.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTACC3 is part of a protein complex that contributes to mitosis. It collaborates with the Aurora A kinase and ch-TOG protein to ensure proper microtubule dynamics and spindle formation. This complex stabilizes kinetochore microtubules by connecting them to the centrosomes allowing accurate chromosome segregation during cell division. TACC3's actions are fundamental to cell cycle regulation and maintaining genomic stability.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe role of TACC3 is linked to the cell cycle regulation and mitosis pathway. It interacts closely with the Aurora A kinase pathway playing a role in regulating centrosome maturation and spindle assembly. In these pathways proteins like TPX2 and PLK1 are also involved working alongside TACC3 to maintain the fidelity of cell division and ensure equal distribution of genetic material to daughter cells.\u003cbr\u003e\nTACC3 is significantly implicated in various cancers. Abnormal expression levels of TACC3 have been associated with the development and progression of glioblastoma and breast cancer. It often exhibits partnerships with FGFR3 leading to oncogenic gene fusions that drive cancer cell growth and survival. Understanding TACC3 and its interactions remains important for developing targeted therapies in oncology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845127098537,"sku":"ab257723","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab257723","provider":"Iright","version":"1.0","type":"link"}