{"product_id":"abcam-ab258000","title":"Abcam, ab258000, Human ILKAP knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nILKAP KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 5 bp deletion in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 5 bp deletion in exon 2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ILKAP, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nILKAP also known as integrin-linked kinase-associated phosphatase is a 43 kDa protein phosphatase. It regulates cell signaling pathways by dephosphorylating specific substrates. It is expressed in various tissues including the heart brain and skeletal muscle. ILKAP specifically interacts with the integrin-linked kinase (ILK) impacting cytoskeletal dynamics and cell adhesion processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe phosphatase activity of ILKAP plays a role in cellular adhesion and movement. ILKAP participates in cellular processes by associating with the ILK complex which includes other key proteins like paxillin and parvin. By modulating the phosphorylation state of substrates within this complex ILKAP influences focal adhesion dynamics and cytoskeletal organization which are critical for maintaining cellular architecture and motility.\u003cbr\u003e\nPathways\u003cbr\u003e\nILKAP acts within the integrin signaling and Wnt signaling pathways. These pathways are important in regulating cell movement differentiation and survival. ILKAP interacts with proteins like β-catenin in the Wnt pathway influencing gene expression critical for cell fate determination. Additionally ILKAP works alongside ILK in the integrin signaling pathway to affect cellular responses like proliferation and migration.\u003cbr\u003e\nILKAP has connections to cancer and cardiovascular diseases. Its regulatory role in cell adhesion and movement links it to cancer metastasis where the altered adhesion leads to the spread of cancer cells. ILKAP also interacts with ILK influencing mechanisms related to tissue remodeling and inflammation in cardiovascular diseases. These associations highlight ILKAP as a potential target for therapeutic intervention in these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845105373353,"sku":"ab258000","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258000","provider":"Iright","version":"1.0","type":"link"}