{"product_id":"abcam-ab258150","title":"Abcam, ab258150, Human PTDSS1 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPTDSS1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PTDSS1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPTDSS1 also known as phosphatidylserine synthase 1 catalyzes the conversion of phosphatidylcholine to phosphatidylserine. This enzyme has a molecular mass of approximately 55 kDa and predominantly resides in the endoplasmic reticulum. It plays a role in lipid metabolism and is expressed in various tissues but is particularly active in the brain and liver. The activity of PTDSS1 is essential for maintaining the balance between different phospholipids in cellular membranes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPhosphatidylserine synthase 1 contributes to the production of phosphatidylserine an important phospholipid found in cell membranes and involved in cell signaling and apoptosis. PTDSS1 is not part of any known complex but regulates phosphatidylserine levels that aid in cellular functions such as lipid signaling and membrane structure. This regulation is essential as disruptions can influence cell function and viability.\u003cbr\u003e\nPathways\u003cbr\u003e\nPTDSS1 is involved in the phospholipid biosynthesis pathway which is important for maintaining the function of cellular membranes. It influences pathways associated with cell survival and apoptosis due to its role in balancing phospholipid composition. PTDSS1 interplays with other proteins in these pathways including kinases and phosphatases which modulate cell signaling and membrane dynamics.\u003cbr\u003e\nPTDSS1 alterations have connections to conditions such as neurological disorders and metabolic syndromes. Disruption in phosphatidylserine levels can contribute to neurodegenerative diseases where PTDSS1 interacts with proteins involved in cell death and survival pathways. Additionally abnormalities in PTDSS1 expression or function might link to metabolic disorders affecting lipid metabolism and cellular energy balance.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855785185449,"sku":"ab258150","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258150","provider":"Iright","version":"1.0","type":"link"}