{"product_id":"abcam-ab258304","title":"Abcam, ab258304, Human ANXA3 (Annexin A3) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nANXA3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 7 bp deletion in exon3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 7 bp deletion in exon3.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ANXA3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAnnexin A3 also known as ANXA3 is a 36 kDa protein that belongs to the annexin family. This family is known for calcium-dependent phospholipid-binding activity. ANXA3 specifically binds to phosphatidylserine a type of phospholipid found in cell membranes. It is expressed in various tissues with notable presence in myeloid cells particularly in the bone marrow and granulocytes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nANXA3 plays roles in membrane-related events including vesicle trafficking and exocytosis. It is not part of a large complex but associates with other proteins depending on its cellular function. ANXA3 can also regulate inflammation by modulating macrophage activation and may influence signal transduction pathways that are linked to immune responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nANXA3 functions in both inflammation and apoptosis. It is closely related to the apoptosis pathway by interacting with signaling molecules like caspases which are critical for the execution of cell death. Additionally through its action in the inflammation pathway ANXA3 is known to interact with proteins such as NF-κB an important regulator of immune and inflammatory responses.\u003cbr\u003e\nAlterations in ANXA3 expression or function associate with various conditions most notably cancer and autoimmune diseases. For instance in certain cancers ANXA3 may contribute to tumor growth and metastasis. In autoimmune diseases its regulation of macrophage activity can misbalance immune responses leading to pathogenesis. Proteins such as ANXA1 and S100A11 also play roles in these pathways and may function along with ANXA3 in disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855790493865,"sku":"ab258304","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258304","provider":"Iright","version":"1.0","type":"link"}