{"product_id":"abcam-ab258322","title":"Abcam, ab258322, Human ATP2C1 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nATP2C1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 4 and 1 bp insertion in exon 4.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 4 and 1 bp insertion in exon 4.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ATP2C1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nATP2C1 also known as Secretory Pathway Calcium ATPase 1 (SPCA1) is an essential calcium pump. It has a molecular mass of approximately 115 kDa. ATP2C1 is located on the Golgi membrane and participates in maintaining calcium and manganese ion homeostasis by transporting these ions from the cytosol into the Golgi apparatus. It shows expression in various tissues with high levels found in epithelial cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe efficient functioning of ATP2C1 regulates calcium ion concentration within the Golgi impacting protein sorting and processing. This protein contributes to cellular signaling and is a part of a broader family of P-type ATPases. Although ATP2C1 does not form part of a complex its activity indirectly influences the function of other calcium-binding proteins through its ion transport role.\u003cbr\u003e\nPathways\u003cbr\u003e\nATP2C1 participates in key calcium homeostasis pathways ensuring cellular functions related to calcium-dependent processes. It plays a role in the intracellular signaling pathway that involves calcium-regulated gene expression. ATP2C1 is functionally linked to proteins such as STIM1 and Orai1 which are critical in the process of calcium reabsorption and signaling.\u003cbr\u003e\nDysfunctional ATP2C1 activity associates with Hailey-Hailey disease a genetic disorder causing recurring blisters and erosions of the skin. Mutations in the ATP2C1 gene result in impaired calcium ion transport contributing to the skin lesions. This condition shows links to the protein desmoglein-1 involved in cell adhesion suggesting disrupted adhesion due to altered calcium ion homeostasis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845188931753,"sku":"ab258322","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258322","provider":"Iright","version":"1.0","type":"link"}