{"product_id":"abcam-ab258354","title":"Abcam, ab258354, Human CERS6 (LASS6) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nCERS6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-CERS6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLASS6 also known as CERS6 is a member of the ceramide synthase family which are enzymes involved in sphingolipid metabolism. CERS6 has a protein mass of approximately 44 kDa. The enzyme is predominantly expressed in the brain liver and kidneys. It operates by catalyzing the formation of ceramides with specific acyl chains playing a role in sphingolipid biosynthesis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCERS6 is involved in the synthesis of longer-chain ceramides which impacts cell membrane structure and signaling. CERS6 performs its function alone and not as part of a larger complex. The enzyme's activity influences cellular processes such as proliferation differentiation and apoptosis contributing to cellular homeostasis. Ceramides produced by CERS6 affect membrane properties and have roles in signal transduction pathways.\u003cbr\u003e\nPathways\u003cbr\u003e\nCERS6 is an important player in the sphingolipid metabolism pathway affecting lipid signaling and apoptosis regulation. This enzyme collaborates with other ceramide synthases such as CERS2 in maintaining balance in ceramide levels influencing cell fate decisions. It also participates in interconnected pathways like the endoplasmic reticulum stress response interacting with proteins such as PERK to modulate apoptosis under stress conditions.\u003cbr\u003e\nCERS6 shows a connection with cancer and neurological disorders. Its altered expression or activity links to tumorigenesis affecting cell growth and survival through interactions with proteins such as p53. In neurological diseases misregulation of CERS6 influences neurodegenerative processes potentially linking to proteins involved in neuroinflammation and cell death. Understanding CERS6's role in these diseases aids in designing therapeutic strategies targeting ceramide metabolism.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855774929065,"sku":"ab258354","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258354","provider":"Iright","version":"1.0","type":"link"}