{"product_id":"abcam-ab258386","title":"Abcam, ab258386, Human CTDSP1 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nCTDSP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 34 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 34 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-CTDSP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCTDSP1 also known as CTD small phosphatase 1 functions as an enzyme that dephosphorylates the C-terminal domain (CTD) of RNA polymerase II. This process regulates transcription elongation and termination. CTDSP1 has a molecular mass of approximately 30 kDa. The protein expresses widely with high levels found in the brain and ovary. It primarily operates within the nucleus reflecting its role in transcription regulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCTDSP1 is involved in gene expression regulation by influencing RNA polymerase II activity. It belongs to a phosphatase family with related proteins like CTDSP2 and CTDSPL sharing overlapping functions. As a part of the CTD phosphatase complex CTDSP1 coordinates with other molecular players to manage gene expression outputs. This regulatory mechanism helps modulate responses to external and internal cellular signals.\u003cbr\u003e\nPathways\u003cbr\u003e\nCTDSP1 takes part in the larger RNA transcription and processing pathways particularly the regulation of RNA polymerase II transcription. CTDSP1 interacts with factors like pre-mRNA splicing components affecting gene expression outcomes. It is associated with pathways that involve Cyclin-dependent kinase 9 (CDK9) which also influences RNA polymerase II activity through phosphorylation events demonstrating its key physiological interactions.\u003cbr\u003e\nCTDSP1 has associations with neurological conditions and cancer. Its involvement in modulating gene expression links it to neurodegenerative disorders like Alzheimer's disease where transcription regulation plays a significant role in disease progression. Additionally changes in CTDSP1 activity relate to tumorigenesis with alterations in CTDSP1 or interacting proteins like CTDSP2 observed in various cancers. This makes CTDSP1 an interesting target for further research into diagnostic and therapeutic applications.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845214687401,"sku":"ab258386","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258386","provider":"Iright","version":"1.0","type":"link"}