{"product_id":"abcam-ab258388","title":"Abcam, ab258388, Human CUTC knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nCUTC KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-CUTC, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCUTC also known as copper homeostasis protein CUTC plays a role in copper transport and homeostasis. It has a molecular mass of approximately 30 kDa. CUTC expression occurs mainly in the liver kidney and intestines suggesting its importance in managing systemic copper levels. CUTC contains several copper-binding motifs that help it in coordinating and potentially transporting copper ions across cellular compartments.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCopper homeostasis protein CUTC functions in the regulation of intracellular copper levels and may interact with copper chaperones. The protein does not work in isolation but instead might form complexes with other copper-binding proteins to facilitate efficient copper regulation. Proper function of CUTC is necessary for various copper-dependent enzymatic activities highlighting its role in maintaining cellular copper sufficiency.\u003cbr\u003e\nPathways\u003cbr\u003e\nCUTC is engaged in the copper homeostasis pathway that ensures adequate copper distribution and detoxification. This protein is associated with other copper-related proteins like ATP7A\/B which transport copper across cellular membranes. Through these interactions CUTC assists in protecting cells from copper toxicity and supports essential biological functions requiring copper.\u003cbr\u003e\nCUTC relates to Wilson's disease and Menkes disease which are disorders of copper metabolism. CUTC interacts with proteins such as ATP7B in Wilson's disease and ATP7A in Menkes disease. Understanding CUTC's function and interactions is key for developing therapeutic strategies aimed at alleviating symptoms associated with impaired copper homeostasis in these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845088039081,"sku":"ab258388","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258388","provider":"Iright","version":"1.0","type":"link"}