{"product_id":"abcam-ab258404","title":"Abcam, ab258404, Human EEF1E1 (AIMP3\/p18) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nEEF1E1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-EEF1E1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAIMP3 also known as p18 or eEF1A1 is a protein that plays an important role in cellular processes. It has a molecular mass of approximately 18 kDa. AIMP3 is expressed ubiquitously meaning it can be found in a variety of tissues throughout the body. As a part of the aminoacyl-tRNA synthetase (ARS) complex AIMP3 contributes to protein translation assisting in the assembly of necessary components for peptide chain formation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe role of AIMP3 extends beyond its contribution to protein synthesis. This protein participates in the macromolecular ARS complex which is essential for accurate and efficient translation of the genetic code into functional proteins. AIMP3 acts as a scaffold stabilizing the assembly and function of the ARS complex. Its involvement is critical for maintaining the fidelity of protein synthesis as errors in this process can lead to cellular dysfunction.\u003cbr\u003e\nPathways\u003cbr\u003e\nAIMP3 engages in several key biological processes. It functions within the DNA damage response pathway where it interacts with proteins such as ATM and ATR to facilitate DNA repair mechanisms. Additionally AIMP3 is involved in the p53 signaling pathway where it contributes to the regulation of cell cycle and apoptosis. This interaction highlights the protein's role in maintaining genomic stability and preventing tumorigenesis.\u003cbr\u003e\nAIMP3 has implications in cancer and neurodegenerative diseases. In cancer downregulation or mutations in AIMP3 can disrupt the DNA repair process leading to genomic instability and tumor development. The protein's interaction with p53 is particularly important as p53 mutations are common in a variety of cancers. Additionally AIMP3 has been associated with neurodegenerative disorders where improper protein synthesis may contribute to disease progression. Its relationship with these conditions highlights the broad impact of AIMP3 on human health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845214261417,"sku":"ab258404","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258404","provider":"Iright","version":"1.0","type":"link"}