{"product_id":"abcam-ab258490","title":"Abcam, ab258490, Human LAMP3 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nLAMP3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon2 and 35 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon2 and 35 bp deletion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-LAMP3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe protein CD208 also known as DC-LAMP or LAMP-3 is integral to the immune system. It has an approximate mass of 60 kDa. CD208 is a type I transmembrane glycoprotein found mainly in the endosomal\/lysosomal compartments of dendritic cells. The expression of CD208 is upregulated during the maturation of dendritic cells making it a reliable marker for these immune cells. Researchers often use CD208 to study dendritic cell biology and its role in immune responses.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCD208 participates in the regulation of the immune response by facilitating antigen processing and presentation. This protein is a part of the lysosomal-associated membrane protein family which contributes to the recycling of MHC class II molecules. Through its involvement in the endocytic pathway CD208 aids in presenting antigens to T lymphocytes an important step in triggering adaptive immunity. The protein colocalizes with MHC class II which is essential for effective antigen presentation.\u003cbr\u003e\nPathways\u003cbr\u003e\nCD208 has a significant role in the endosomal-lysosomal pathway where it interacts with other proteins involved in antigen processing. It also engages in the intracellular trafficking pathway that affects the presentation of peptides on MHC class II molecules. The interaction with proteins such as HLA-DR is vital for efficient antigen presentation to helper T cells. CD208 supports the proper delivery of peptide-loaded MHC molecules to the cell surface a necessity for a functional adaptive immune response.\u003cbr\u003e\nCD208 has implications in autoimmune diseases and infectious diseases. Its expression is notably altered in conditions like lupus and certain infectious diseases. In systemic lupus erythematosus (SLE) abnormal CD208 expression correlates with the dysregulation of immune responses. Additionally during infectious diseases changes in CD208 expression can impact the activation and function of dendritic cells affecting disease progression. The interaction of CD208 with proteins like MHC class II molecules highlights its role in disease pathology and immune regulation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845177233577,"sku":"ab258490","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258490","provider":"Iright","version":"1.0","type":"link"}