{"product_id":"abcam-ab258523","title":"Abcam, ab258523, Human MPI (Mannose Phosphate Isomerase) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nMPI KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp insertion in exon 3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp insertion in exon 3.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MPI, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMannose Phosphate Isomerase also known as PMI is an enzyme that catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate. This enzyme with a mass of approximately 42 kDa plays an important role in the metabolism of mannose a type of sugar. Expression of Mannose Phosphate Isomerase occurs in many tissues including liver muscle and fibroblasts where it helps maintain the balance of sugar phosphates needed for various cellular functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis enzyme facilitates the processing of mannose through the biosynthesis pathways of glycoproteins and glycolipids. Mannose Phosphate Isomerase is not part of a larger protein complex but it functions independently to execute its role within the cell. It ensures that cells have adequate mannose-6-phosphate which is essential for proper glycosylation a process necessary for protein folding and stability.\u003cbr\u003e\nPathways\u003cbr\u003e\nMannose Phosphate Isomerase influences the hexosamine biosynthesis pathway and glycolysis. In the hexosamine biosynthesis pathway it works closely with enzymes such as phosphomannomutase to provide critical components for the production of glycosaminoglycans and glycoproteins. In glycolysis it helps integrate mannose into energy production processes by providing intermediates necessary for glucose metabolism.\u003cbr\u003e\nDefects in Mannose Phosphate Isomerase can lead to conditions such as congenital disorder of glycosylation type Ib and hypoglycemia-related disorders. This enzyme's malfunction disrupts normal glycosylation which is important for protein function and stability linking it to glycosylation disorders. Connections also exist with phosphomannomutase another enzyme affected in similar glycosylation disorders highlighting its relevance in disease pathology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845170024617,"sku":"ab258523","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258523","provider":"Iright","version":"1.0","type":"link"}