{"product_id":"abcam-ab258597","title":"Abcam, ab258597, Human PPM1B knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPPM1B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 3.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PPM1B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPPM1B also known as protein phosphatase 1B is a serine\/threonine phosphatase with a molecular mass of approximately 55 kDa. This protein plays an important role in dephosphorylating serine\/threonine residues on its target proteins adjusting their functional states. PPM1B is widely expressed in various tissues throughout the body including the heart skeletal muscle and liver. Its activity helps in modulating different signaling pathways by reversing the phosphorylation status of target proteins.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPPM1B influences diverse cellular processes such as cell cycle regulation and stress response. It is not part of a large complex but interacts with specific substrates to exert its effects. PPM1B can impact apoptosis and cell differentiation through its action on certain substrates. Its regulatory function is important for maintaining cellular homeostasis and adaptation to environmental changes.\u003cbr\u003e\nPathways\u003cbr\u003e\nMultiple signaling cascades demonstrate the involvement of PPM1B. It plays a significant role in the regulation of the p38 MAPK pathway which is important for stress response and inflammatory processes. In this pathway PPM1B interacts with MAPKAPK2 modulating its activity. PPM1B also participates in the TGF-beta signaling pathway where it can affect the phosphorylation status of SMADs. These interactions emphasize PPM1B's regulatory importance in response to extracellular signals.\u003cbr\u003e\nAlterations in PPM1B activity are linked to cardiovascular and inflammatory conditions. For example changes in its function can contribute to the pathogenesis of hypertension through its effect on the TGF-beta pathway and related proteins like SMAD2. In inflammatory diseases the role of PPM1B in the p38 MAPK pathway shows its connection to proteins such as MAPKAP2 highlighting its potential involvement in inflammatory responses. These connections demonstrate the therapeutic interest in targeting PPM1B for disease treatment and management.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847388549289,"sku":"ab258597","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258597","provider":"Iright","version":"1.0","type":"link"}