{"product_id":"abcam-ab258741","title":"Abcam, ab258741, Human TUBB2A knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTUBB2A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 10 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 10 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TUBB2A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTUBB2A also known as tubulin beta-2A chain is an important component of microtubule structures which are integral for cell shape intracellular transport and division. The protein has a molecular mass of approximately 49 kDa and forms the heterodimer with alpha-tubulin. These heterodimers further polymerize to create microtubules. TUBB2A expression is widespread showing high levels in neuronal tissues where microtubule dynamics play a big role in proper neuronal function and development.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTUBB2A plays a central part in the polymerization and depolymerization of microtubules which are essential processes in mitosis and meiosis. As part of the tubulin superfamily TUBB2A associates closely with other tubulin isoforms to form dynamic microtubule structures. These structures undergo rapid assembly and disassembly which are critical for chromosome segregation during cell division and for intracellular transport in axons.\u003cbr\u003e\nPathways\u003cbr\u003e\nTUBB2A functions within the microtubule dynamics pathway and is importantly involved in the mitotic spindle assembly ensuring accurate chromosome segregation. This protein interacts with motor proteins like kinesins and dyneins which facilitate cellular transport and mitotic processes. Additionally it participates in the neuronal signaling pathways interacting with tau and other associated proteins involved in microtubule stability and synaptic function.\u003cbr\u003e\nTUBB2A mutations have been linked to lissencephaly a rare brain malformation characterized by a smooth cerebral surface and developmental delays. These mutations disrupt microtubule stability affecting neuronal migration. Additionally neurodegenerative diseases such as Alzheimer's have demonstrated connections to TUBB2A through interactions with tau protein which is known to form pathological aggregates in these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845178151081,"sku":"ab258741","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258741","provider":"Iright","version":"1.0","type":"link"}