{"product_id":"abcam-ab258773","title":"Abcam, ab258773, Human ABCF2 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nABCF2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 2 and 5 bp deletion in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 2 and 5 bp deletion in exon 2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ABCF2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe human ABCF2 protein also known as ATP-binding cassette sub-family F member 2 belongs to the ABC transporter superfamily. Weighing approximately 85 kDa ABCF2 does not transport molecules across membranes like most other members of this family. Instead it participates in regulating translation and is involved in cellular stress responses. Researchers find ABCF2 expression in various tissues including liver lung and muscle. It does not span membranes and it anchors within the cytoplasm of cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nABCF2 impacts translation regulation and stress response through different cellular processes. This protein functions independently and may also participate in complexes with other proteins. Through mechanisms not fully understood ABCF2 associates with cellular stress granules that form in response to environmental stress. These interactions suggest roles in protein synthesis regulation during stress which indirectly influences cell survival and adaptation.\u003cbr\u003e\nPathways\u003cbr\u003e\nABCF2 integrates into cellular processes related to stress and translation. It intersects with pathways like eukaryotic initiation factor 2 (EIF2) signaling which helps control translation initiation during stress. ABCF2 shares pathway interactions with proteins such as EIF2B and other ribosomal elements indicating a connection to translation regulation. The involvement in ribosomal function places ABCF2 within important cellular response networks especially under conditions that affect protein synthesis fidelity.\u003cbr\u003e\nABCF2 links to specific pathologies primarily through stress response mechanisms. Cancer particularly hepatic and pulmonary types shows associations with dysregulated ABCF2 expression possibly affecting tumor growth and chemoresistance. Researchers note potential interactions with heat shock proteins which share pathways implicated in cancer cell survival. Additionally preliminary evidence suggests ABCF2 roles in neurological disorders although the connections and interactions with other proteins need further study.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845074899113,"sku":"ab258773","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258773","provider":"Iright","version":"1.0","type":"link"}