{"product_id":"abcam-ab258794","title":"Abcam, ab258794, Human BRK1 (C3orf10) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nBRK1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1 and 4 bp deletion in exon1 and 88 bp insertion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1 and 4 bp deletion in exon1 and 88 bp insertion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-BRK1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nC3orf10 also known as ARMH4 or Armadillo Repeat Containing 4 encodes a protein with a currently limited mechanical description. This protein weighs approximately 54 kDa and is expressed in multiple tissues notably in the brain and skeletal muscle. Studies indicate it may play a role in cellular architecture or signal transduction but exact mechanical roles require further investigation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe protein product of C3orf10 may participate in cellular structural organization or intracellular signaling. Evidence suggests it functions as part of a larger protein complex though not extensively characterized. It possibly assists in linking cellular components to ensure structural integrity or facilitate signaling processes in cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nC3orf10 integrates into certain cellular pathways potentially involving cytoskeleton organization or signaling cascades. It associates with proteins involved in structural support and signal relay including some members of the armadillo family known for roles in cell adhesion and signaling. However the precise pathways C3orf10 participates in remain to be fully elucidated.\u003cbr\u003e\nC3orf10 holds interest in research related to neurodegenerative conditions and muscular disorders. Alterations in its expression or function may link to conditions like amyotrophic lateral sclerosis (ALS) or dystrophin-related muscle dysfunction. C3orf10 could interact with proteins such as dystrophin or others involved in neurodegeneration highlighting its potential impact on disease pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845175595177,"sku":"ab258794","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258794","provider":"Iright","version":"1.0","type":"link"}