{"product_id":"abcam-ab258827","title":"Abcam, ab258827, Human COX8A knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nCOX8A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-COX8A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCOX8A also known as Cytochrome c oxidase subunit 8A is a component of the cytochrome c oxidase complex which plays a vital role in the electron transport chain within mitochondria. This protein has a molecular mass of approximately 8.7 kDa. COX8A is highly expressed in tissues with high energy demands such as muscle and brain where efficient ATP production is needed.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCOX8A is an integral part of the large cytochrome c oxidase complex also known as Complex IV in the mitochondrial inner membrane. This complex is involved in oxidative phosphorylation where it catalyzes the reduction of oxygen to water a final step of the electron transport chain. COX8A contributes to the correct assembly and stability of the cytochrome c oxidase complex ensuring proper mitochondrial function and energy production.\u003cbr\u003e\nPathways\u003cbr\u003e\nCOX8A participates in the oxidative phosphorylation pathway and is an important player in the process that produces ATP from ADP and inorganic phosphate. It functions together with other subunits in Complex IV such as COX2 and COX3 to facilitate efficient electron transfer and proton pumping. Through its activity in these processes COX8A directly influences cellular respiration and energy metabolism which are critical for cellular homeostasis.\u003cbr\u003e\nCOX8A has connections to mitochondrial disorders such as Leigh syndrome and cytochrome c oxidase deficiency. These conditions arise from defects in mitochondrial oxidative phosphorylation affecting tissues with high metabolic demands. Alterations in COX8A along with other complex IV components like COX2 can contribute to the pathogenesis of these diseases as they disrupt ATP production and lead to energy deficits in affected cells.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845218750633,"sku":"ab258827","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258827","provider":"Iright","version":"1.0","type":"link"}