{"product_id":"abcam-ab258882","title":"Abcam, ab258882, Human GHITM (MICS1) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nGHITM KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2 and 4 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2 and 4 bp deletion in exon2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-GHITM, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMICS1 also known as mitochondrial import inner membrane translocase subunit 13 is a protein that plays a role in the transport of polypeptides across the mitochondrial inner membrane. It has an approximate mass of 13 kDa. You can find MICS1 expression predominantly in tissues with high-energy demands such as muscle and brain tissues where efficient mitochondrial function is critical.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMICS1 contributes to the establishment of the mitochondrial inner membrane potential essential for maintaining proper mitochondrial function. MICS1 forms part of the translocase of inner membrane (TIM) complex which facilitates the import of preproteins into the mitochondria. This activity supports cellular energy metabolism by ensuring efficient electron transport chain operation and ATP production.\u003cbr\u003e\nPathways\u003cbr\u003e\nMICS1 is involved in the mitochondrial protein import pathway and the maintenance of mitochondrial membrane potential. It interacts with other components of the TIM complex such as Tim23 and works closely with proteins in the oxidative phosphorylation pathway. These interactions ensure a seamless integration of imported mitochondrial proteins into the inner membrane facilitating cellular energy production processes.\u003cbr\u003e\nMICS1 has links to conditions related to mitochondrial dysfunction such as mitochondrial myopathy and Leigh syndrome. Dysregulation of MICS1 may disrupt mitochondrial protein import and membrane potential maintenance contributing to these conditions. Its interaction with other mitochondrial proteins like Tim23 further associates it with the broader mitochondrial dysfunction often present in these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845188735145,"sku":"ab258882","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258882","provider":"Iright","version":"1.0","type":"link"}