{"product_id":"abcam-ab258897","title":"Abcam, ab258897, Human HENMT1 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nHENMT1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 3.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-HENMT1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHENMT1 also known as HEN1 methyltransferase homolog 1 is an enzyme with a molecular mass of approximately 55 kDa. It is expressed in the germ cells of both mammals and plants. This enzyme belongs to the small RNA methylation family and it plays an important role in the modification of small RNAs specifically piwi-interacting RNAs (piRNAs). HENMT1's primary function is transferring a methyl group to the 3' end of piRNAs protecting them against uridylation and exonucleolytic degradation keeping their integrity and stability necessary for their function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme HENMT1 participates in piRNA biogenesis. These piRNAs are part of a larger ribonucleoprotein complex that includes Piwi proteins which are essential for transposon silencing in the germline. This complex ensures the epigenetic and post-transcriptional silencing of transposable elements consequently protecting genomic integrity. HENMT1's activity is fundamental for safeguarding the genome from potential aberrations caused by active transposons.\u003cbr\u003e\nPathways\u003cbr\u003e\nHENMT1 plays a significant part in the piRNA pathway which is important for gene silencing in the germline. Through this pathway it contributes to the protection of the genome against transposable elements by working alongside proteins like Piwi. The RNA interference (RNAi) pathway also involves HENMT1 although indirectly as RNAi and piRNA pathways share some mechanistic similarities in their mode of silencing unwanted genetic elements.\u003cbr\u003e\nHENMT1 is connected to male infertility and certain cancers. Defects or malfunctions in HENMT1 can disrupt piRNA production leading to genomic instability and contributing to infertility in males. Additionally irregularities in its function might link it to cancer development possibly through faulty regulation of transposons. Other proteins like Piwi are relevant to understanding HENMT1's involvement in these conditions due to their collaborative function in the piRNA complex.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847387304105,"sku":"ab258897","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258897","provider":"Iright","version":"1.0","type":"link"}