{"product_id":"abcam-ab258941","title":"Abcam, ab258941, Human LARP4B knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nLARP4B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 149 bp insertion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 149 bp insertion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-LARP4B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLARP4B also known as La-related protein 4B is a protein of approximately 78 kDa. It is expressed in various tissues but shows higher expression in testis and liver. The protein contains an RNA-binding domain which indicates its role in RNA metabolism. LARP4B interacts with mRNA influencing its stability and translation. This protein belongs to the LARP family which is known for involvement in RNA-binding processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nLARP4B plays a role in regulating mRNA stability and translation. It often cooperates with mRNA decay and translation initiation factors. LARP4B does not form a stable complex but associates transiently with other RNA-binding proteins to perform its function. Its ability to bind mRNA suggests involvement in gene expression post-transcriptionally impacting various cellular processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nLARP4B is implicated in the mTOR signaling pathway and the RNA processing pathway. The mTOR pathway is essential for cell growth and proliferation linking nutrient availability to these processes. LARP4B works in tandem with other proteins like LARP1 and EIF4E to modulate mRNA stability indirectly influencing cell growth through translation regulation.\u003cbr\u003e\nLARP4B has a connection to cancer and neurological disorders. Abnormal expression of LARP4B has been observed in certain cancers suggesting a role in tumorigenesis. In neurological disorders differences in LARP4B expression levels have been associated with disease progression. The protein establishes a relationship with LARP1 in cancer contexts further exploring how altered mRNA translation contributes to these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847384420521,"sku":"ab258941","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258941","provider":"Iright","version":"1.0","type":"link"}