{"product_id":"abcam-ab258962","title":"Abcam, ab258962, Human MFSD1 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nMFSD1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MFSD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMFSD1 also known as Major Facilitator Superfamily Domain Containing 1 functions as a transmembrane protein that facilitates transport across cell membranes. It displays a mass of approximately 55 kDa. MFSD1 is expressed in various tissues with notable presence in the brain liver and kidney. It localizes primarily at the lysosome suggesting its role in intracellular transport and homeostasis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMFSD1 acts as a transporter within the lysosomal membrane. It might be a part of larger lysosomal membrane complexes involved in nutrient sensing and regulation. MFSD1 contributes to maintaining cellular homeostasis by transporting small molecules across the lysosomal membrane. The precise substrates of MFSD1 remain under investigation but they likely include metabolites that are important for cellular function.\u003cbr\u003e\nPathways\u003cbr\u003e\nMFSD1 operates within lysosomal transport pathways and metabolic regulation. It is associated with nutrient-sensing pathways that are essential for energy homeostasis. MFSD1 interacts with other metabolic regulatory proteins such as mTOR that play a role in cellular growth and metabolic processes. These interactions link MFSD1 to important pathways that regulate cellular metabolism and response to nutrient availability.\u003cbr\u003e\nMFSD1 has implications in neurodegenerative conditions and metabolic disorders. Alterations in MFSD1 function may contribute to lysosomal storage disorders as it affects lysosomal transport efficiency. Additionally its interaction with mTOR relates MFSD1 to disorders like neurodegeneration where disrupted cellular homeostasis plays a significant role. Studying MFSD1 can enhance understanding of its role in diseases and offer potential therapeutic targets.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855777255593,"sku":"ab258962","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258962","provider":"Iright","version":"1.0","type":"link"}