{"product_id":"abcam-ab258963","title":"Abcam, ab258963, Human MKRN1 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nMKRN1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon 1 and 30 bp deletion in exon 1 and 3 bp deletion in exon 1 and 5 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon 1 and 30 bp deletion in exon 1 and 3 bp deletion in exon 1 and 5 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MKRN1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMKRN1 also known as Makorin-1 is a protein that functions as an E3 ubiquitin ligase. This protein has a molecular mass of approximately 58-60 kDa. It is highly expressed in human tissues including the brain heart and skeletal muscle. MKRN1 contains distinct zinc finger motifs that allow it to tether to its substrates aiding in target protein modification and degradation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMKRN1 regulates protein stability and turnover within cells. It plays a significant role in controlling gene expression through ubiquitination processes. MKRN1 does not form part of larger protein complexes but interacts individually with its targets. Researchers study its impact on the transcriptional activities of specific proteins given its ability to tag substrates for proteasomal degradation.\u003cbr\u003e\nPathways\u003cbr\u003e\nMKRN1 participates in key cellular pathways such as cell cycle regulation and apoptosis. It interacts with proteins like p53 and ribosomal protein L6 influencing these pathways. Its ubiquitin ligase function connects it to various cellular control mechanisms essential for maintaining homeostasis and responding to cellular stressors.\u003cbr\u003e\nResearchers established a connection between MKRN1 and cancer specifically prostate cancer. Its regulation of p53 activity influences tumor growth and cell death. Additionally studies show links between MKRN1 and neurodegenerative disorders implicating its role in protein aggregation and neuron survival. MKRN1's interaction with key proteins such as p53 and neuronal factors highlights its potential as a therapeutic target in these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845179068585,"sku":"ab258963","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258963","provider":"Iright","version":"1.0","type":"link"}