{"product_id":"abcam-ab258976","title":"Abcam, ab258976, Human MTHFD1L knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nMTHFD1L KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2 and 8 bp deletion in exon2 and Insertion of the selection cassette in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2 and 8 bp deletion in exon2 and Insertion of the selection cassette in exon2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MTHFD1L, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMTHFD1L also known as methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like functions as an enzyme participating in folate metabolism. With an approximate mass of 105 kDa MTHFD1L is active in the mitochondria. This enzyme facilitates the interconversion of tetrahydrofolate derivatives supporting the folate-mediated one-carbon metabolic pathway. MTHFD1L shows expression in various tissues with a noticeable presence in liver and embryonic tissues where it has roles in embryogenesis and tissue-specific functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMTHFD1L catalyzes reactions converting methylene-THF to methenyl-THF and then to formyl-THF utilizing NADP+ as a cofactor. It does not function as a standalone component; instead it may operate as part of a larger complex engaged in one-carbon metabolism. Its activity contributes to the synthesis of important biomolecules such as nucleotides and amino acids emphasizing its role in DNA synthesis and repair.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe folate-mediated one-carbon metabolism uses MTHFD1L to manage one-carbon units. This enzyme's function fits into the folate pathway important for nucleotide biosynthesis. It connects to other proteins such as MTHFD1 and SHMT1 which also partake in folate metabolism supporting interconnected biochemical pathways in various cellular processes.\u003cbr\u003e\nDefects or alterations in MTHFD1L are linked to neural tube defects and some cancers. The enzyme's involvement in embryonic development and DNA repair suggests that mutations or dysregulation may contribute to these conditions. MTHFD1 and SHMT1 related proteins have similar associations highlighting their collective impact on disease when there is impaired folate metabolism.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845178282153,"sku":"ab258976","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab258976","provider":"Iright","version":"1.0","type":"link"}