{"product_id":"abcam-ab259035","title":"Abcam, ab259035, Human PCOLCE2 (PCPE-2) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPCOLCE2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 14 bp deletion in exon1 and 16 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 14 bp deletion in exon1 and 16 bp deletion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PCOLCE2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPCPE-2 also known as procollagen C-endopeptidase enhancer 2 enhances the activity of procollagen C-proteinase. It weighs approximately 42 kDa. PCPE-2 is expressed in various tissues including liver and kidney. This protein influences collagen biosynthesis by binding to substrates and increasing enzymatic activity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPCPE-2 assists in the maturation of type I II and III procollagens. It does not form a part of any larger complex but it increases the cleavage efficiency of the C-terminal propeptide from procollagens. By promoting collagen fibril formation PCPE-2 plays a role in tissue organization and structure.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe role of PCPE-2 in collagen biosynthesis connects it to the extracellular matrix organization pathway. Additionally this protein interacts with enzymes such as bone morphogenetic protein 1 (BMP1) which are important for collagen maturation. These interactions facilitate the assembly and structure of the extracellular matrix.\u003cbr\u003e\nPCPE-2's involvement in collagen maturation links it to fibrosis a condition characterized by excessive collagen deposition. It also relates to osteogenesis imperfecta a disorder affecting bone strength. In these conditions PCPE-2 works alongside proteins like BMP1 influencing collagen processing and accumulation thereby affecting tissue characteristics.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845167730857,"sku":"ab259035","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab259035","provider":"Iright","version":"1.0","type":"link"}