{"product_id":"abcam-ab259060","title":"Abcam, ab259060, Human PQBP1 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPQBP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 28 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 28 bp deletion in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PQBP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPQBP1 also known as Polyglutamine-Binding Protein 1 is a protein that plays an important role in cellular functions. Its molecular mass is approximately 34 kDa. PQBP1 is expressed widely in human tissues with higher levels detected in the brain and muscle. It contains a WW domain which facilitates its interaction with other proteins through proline-rich sequences impacting various cellular processes such as transcriptional regulation and RNA splicing.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPQBP1 interacts with other proteins through its ability to bind to polyglutamine tracts. This protein often participates as a subunit in large complexes influencing the transcriptional machinery. It plays a role by regulating gene expression and maintaining proper RNA splicing which are critical for normal cell function and development. The ability to form complexes enables PQBP1 to impact multiple pathways critical for cellular processes highlighting its functional versatility.\u003cbr\u003e\nPathways\u003cbr\u003e\nPQBP1 actively participates in the RNA splicing pathway and transcription regulation. It interacts closely with the WNT signaling pathway which is vital for cellular proliferation and differentiation. Within these pathways PQBP1 associates with proteins like ATXN1 and SMN facilitating its role in gene expression modulation and maintaining cellular homeostasis.\u003cbr\u003e\nPQBP1 mutations are linked to neurological conditions such as Renpenning syndrome and intellectual disability. These mutations can alter the PQBP1's interaction with other proteins including those in the WNT pathway potentially leading to disrupted cellular functions. The protein ATXN1 connected through disease pathways interacts with PQBP1 amplifying the effects of its mutations and contributing to the pathology of neurodegenerative diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855787249833,"sku":"ab259060","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab259060","provider":"Iright","version":"1.0","type":"link"}