{"product_id":"abcam-ab259094","title":"Abcam, ab259094, Human RTP4 knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRTP4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 5 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 5 bp deletion in exon2.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RTP4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRTP4 also known as Receptor Transporter Protein 4 plays a significant role in the endoplasmic reticulum where it facilitates the trafficking and proper function of olfactory receptors. It has a molecular mass of approximately 31 kDa. RTP4 expression is mainly found in the olfactory sensory neurons but also in various other tissues such as the brain and liver. It helps in guiding G protein-coupled receptors (GPCRs) to the cell membrane ensuring their functional expression.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe RTP4 protein aids in the transport and functional expression of GPCRs without forming part of any known stable complexes. This chaperone activity is vital for GPCR function impacting signal transduction by promoting proper receptor folding and surface expression. RTP4 ensures that olfactory and other related receptors can adequately respond to stimuli. Its chaperone activity is important for maintaining GPCR dynamics in various cell types.\u003cbr\u003e\nPathways\u003cbr\u003e\nRTP4 significantly influences GPCR signaling pathways and olfactory signaling pathways. It directly affects the transport and activation of olfactory receptors key components in the olfactory signaling pathway. RTP4's interaction with olfactory receptors impacts the downstream components such as adenylyl cyclase leading to successful signal transduction. Additionally RTP4 interacts with proteins in GPCR signaling that modify receptor availability on the cell surface.\u003cbr\u003e\nRTP4 has implications in anosmia the loss or impairment of the sense of smell and potentially in liver-related disorders. Disruption of RTP4 function can lead to defective olfactory receptor trafficking resulting in anosmia. RTP4-related pathways implicate proteins like OR51E1 in olfactory dysfunctions. There may be a less clear association with liver disorders involving misfolded GPCRs; however this link requires further exploration.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847388614825,"sku":"ab259094","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab259094","provider":"Iright","version":"1.0","type":"link"}