{"product_id":"abcam-ab259097","title":"Abcam, ab259097, Human SCAF11 (SFRS2IP) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSCAF11 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon10 and 2 bp deletion in exon10.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon10 and 2 bp deletion in exon10.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SCAF11, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSFRS2IP also known as SFRS2-interacting protein and Cisplatin Resistance-Associated Overexpressed Protein (CRISP-1) is a protein with a relative mass of around 92 kilodaltons. It primarily interacts with the serine\/arginine-rich splicing factor 2 (SFRS2) as indicated by its name. Researchers have found SFRS2IP mainly in the nucleus of cells where it plays a significant role in pre-mRNA splicing. Studies show its expression in various tissues but it is especially present in actively dividing cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nProteins like SFRS2IP contribute to the regulation of RNA processing and stability. SFRS2IP participates in the spliceosome complex facilitating correct splicing and maturation of precursor mRNA into mature RNA. This process is essential for gene expression regulation ensuring that proteins are synthesized properly and in necessary amounts. SFRS2IP's involvement in these processes influences cell cycle progression and can impact cellular responses to environmental changes.\u003cbr\u003e\nPathways\u003cbr\u003e\nSeveral studies have included SFRS2IP in processes related to mRNA splicing and gene expression. It is closely connected to the spliceosome pathway where it cooperates with SFRS2 and other splicing factors contributing to proper intron removal and exon joining in precursor mRNA. The protein also interacts with components of the RNA transport pathway ensuring the correct delivery of mRNA to the ribosome for translation.\u003cbr\u003e\nScientists have linked SFRS2IP to cancer and drug resistance mechanisms. Elevated expression levels of this protein have been observed in certain neoplastic tissues suggesting a role in tumorigenesis. It is associated in pathways involving oncogenic proteins like SFRS2 which influence cell proliferation and survival. Additionally studies have highlighted a connection between this protein and cisplatin resistance where SFRS2IP may interact with DNA repair proteins to modulate the cellular response to chemotherapy.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845129162921,"sku":"ab259097","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab259097","provider":"Iright","version":"1.0","type":"link"}