{"product_id":"abcam-ab259107","title":"Abcam, ab259107, Human SGTA knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSGTA KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 22 bp deletion in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 22 bp deletion in exon 2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SGTA, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSGTA also known as Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha is a co-chaperone protein with a molecular mass of approximately 35 kDa. It plays a role in the protein quality control system by interacting with misfolded proteins. SGTA has a TPR domain essential for binding with other chaperones and client proteins. You can find this protein expressed in many tissues with highest levels in the liver kidney and testis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSGTA influences protein folding and degradation impacting cellular protein homeostasis. It functions as part of the chaperone system and forms complexes with Hsp70 and Hsp90 helping in stabilizing and refolding damaged proteins. SGTA's interaction with steroid hormone receptors has roles in regulating their stability and function. It also interacts with other cellular proteins indicating its diverse biological responsibilities.\u003cbr\u003e\nPathways\u003cbr\u003e\nSmall glutamine-rich tetratricopeptide repeat-containing protein alpha integrates into cellular mechanisms such as the ER-associated degradation (ERAD) and the ubiquitin-proteasome pathway. It associates with proteins like BAG6 which targets mislocalized proteins for degradation. It helps guide the quality control process in cells allowing the removal of faulty proteins and supporting cellular health.\u003cbr\u003e\nSGTA shows associations with neurodegenerative diseases and certain cancers. It links to the accumulation of misfolded proteins which are common in disorders like Alzheimer's disease. In cancer SGTA's relationship with steroid hormone receptors can affect tumor growth and progression. Through pathways involving the androgen receptor SGTA potentially influences prostate cancer development.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855786430633,"sku":"ab259107","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab259107","provider":"Iright","version":"1.0","type":"link"}