{"product_id":"abcam-ab259132","title":"Abcam, ab259132, Human SLC39A1 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSLC39A1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 3 and Insertion of the selection cassette in exon 3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 3 and Insertion of the selection cassette in exon 3.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SLC39A1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSLC39A1 also known as ZIP1 is a zinc transporter protein that facilitates the influx of zinc ions into cells. This protein consists of 324 amino acids resulting in a molecular mass of approximately 34.26 kDa. SLC39A1 is expressed in various tissues throughout the body with high levels observed in the prostate pancreas and other zinc-related tissues. The protein belongs to the ZIP family of metal ion transporters which is key in maintaining intracellular zinc homeostasis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe SLC39A1 protein plays an essential role in cellular zinc uptake by increasing cytoplasmic zinc concentrations. This protein is not part of a larger complex but interacts with zinc ions directly ensuring adequate zinc availability for numerous cellular processes. Zinc is a critical cofactor for many enzymes and is necessary for proper immune function protein synthesis and cellular proliferation. By regulating zinc levels in cells SLC39A1 supports diverse physiological functions and maintains cellular health.\u003cbr\u003e\nPathways\u003cbr\u003e\nSLC39A1 participates in zinc homeostasis and signal transduction pathways. It is pivotal in the regulation of the metallothionein expression pathway which helps detoxify heavy metals and protect against oxidative stress. Also SLC39A1 interacts with the NF-κB signaling pathway where zinc acts as a second messenger influencing cell survival and immune response. Proteins such as MT (metallothionein) and IκBα interact with SLC39A1 in these pathways thereby linking zinc transport to broader cellular functions and responses.\u003cbr\u003e\nDisruptions in SLC39A1 function have been linked to prostate cancer and neurodegenerative diseases. In prostate cancer altered zinc transport by SLC39A1 is associated with reduced cellular zinc levels contributing to tumorigenesis. The reduction in zinc affects various proteins involved in prostate cancer progression including the androgen receptor. In neurodegenerative diseases like Alzheimer's dysfunctional zinc homeostasis related to abnormal SLC39A1 activity leads to increased oxidative stress and contributes to neuronal damage. Understanding the role of SLC39A1 in these conditions is important for developing targeted therapeutic strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845132570793,"sku":"ab259132","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab259132","provider":"Iright","version":"1.0","type":"link"}