{"product_id":"abcam-ab259151","title":"Abcam, ab259151, Human SPAG5 (Astrin\/Deepest) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSPAG5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 19 bp deletion in exon 2 and 2 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 19 bp deletion in exon 2 and 2 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SPAG5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAstrin also known as SPAG5 or Deepest is a protein important for cell division. It has a molecular mass of approximately 45 kDa. Researchers find Astrin primarily in the cytosol and preferably expressed in proliferating cells. Astrin plays a significant role in spindle stabilization during mitosis binding to microtubules to ensure proper chromosome segregation. This protein has become a focus of study due to its critical functions during cell cycle progression.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAstrin facilitates kinetochore-microtubule attachment participating in the mitotic spindle assembly complex. This complex ensures chromosome alignment and segregation which is critical in cellular replication. Since precise regulation of this process is necessary for genetic stability Astrin's function is indispensable. Its presence influences the structural integrity of dividing cells linking it to the stability of genetic information passed to daughter cells after division.\u003cbr\u003e\nPathways\u003cbr\u003e\nAstrin operates within the spindle assembly checkpoint and cell cycle pathways. The spindle assembly checkpoint pathway ensures that cells do not progress to anaphase until all chromosomes are properly attached to the spindle apparatus. In synergy with proteins like Ndc80 and KNL1 Astrin maintains attachment integrity. Its interactions with these proteins ensure accurate chromosome segregation protecting against chromosomal instability which can lead to aneuploidy.\u003cbr\u003e\nAstrin's role connects it to conditions like cancer and developmental disorders. Overexpression of Astrin has associations with tumor progression since its function affects cell proliferation. In the context of cancer proteins like Aurora B also influence Astrin's activity. The misregulation of this pathway can lead to faulty chromosomal alignment and segregation contributing to oncogenesis. Such insights make Astrin a potential biomarker and therapeutic target in cancer research.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845129785513,"sku":"ab259151","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab259151","provider":"Iright","version":"1.0","type":"link"}