{"product_id":"abcam-ab259157","title":"Abcam, ab259157, Human SSR4 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSSR4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon 1 and 8 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon 1 and 8 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SSR4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSSR4 also known as TRG4 or CDG1S is a subunit of the signal recognition particle receptor responsible for the translocation of proteins across the membrane of the endoplasmic reticulum. With an approximate mass of 24 kDa SSR4 is expressed predominately in tissues with high secretory functions such as the liver and pancreas. It plays an important role in facilitating the proper insertion and folding of nascent polypeptide chains by cooperating with other components of the translocation machinery.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSSR4 functions as part of the signal sequence receptor (SSR) complex. This complex is essential for the targeting and translocation of newly synthesized proteins into the endoplasmic reticulum lumen. SSR4 interacts directly with signal sequences of nascent polypeptides to ensure their correct orientation and localization during protein synthesis. Proper functioning of SSR4 is necessary for the homeostasis of the cellular secretory pathway.\u003cbr\u003e\nPathways\u003cbr\u003e\nSSR4 is involved in the protein translocation pathway which is essential for protein sorting and trafficking. This pathway intersects with the unfolded protein response (UPR) pathway which maintains protein homeostasis under stress conditions. In these pathways SSR4 collaborates with proteins like Sec61 to mediate translocation and assist in protein folding. Disruptions in these pathways can lead to cellular dysfunction and stress-related responses.\u003cbr\u003e\nDefects or alterations in SSR4 are linked to congenital disorders of glycosylation (CDGs) which affect multiple organ systems. Altered glycoprotein production in these disorders results from impaired protein translocation demonstrating SSR4’s influence on glycoprotein processing. Furthermore SSR4 may interact with proteins like glycosyltransferases affecting their function in cellular processes and contributing to the broad clinical spectrum observed in CDGs.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845175726249,"sku":"ab259157","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab259157","provider":"Iright","version":"1.0","type":"link"}